Fig. 6: MiR-451 upregulates miR-185 at the post-transcriptional level by directly targeting XPO-1 in LX-2 cells. | Cell Death & Disease

Fig. 6: MiR-451 upregulates miR-185 at the post-transcriptional level by directly targeting XPO-1 in LX-2 cells.

From: Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells

Fig. 6

a RT-qPCR analysis of pri-miR-185, pre-miR-185 and miR-185. b, c Western blotting analysis of XPO-1. d Putative binding sites (red font) of miR-451 were predicted in the 3′UTR of XPO-1 mRNA. The vertical line represents complementary base pairs between miR-451 and XPO-1 mRNA, and the gray shading indicates the seed sequence of miR-451. e Dual luciferase reporter assay was performed to examine the binding between miR-451 and the 3′UTR of XPO-1 mRNA. HEK 293T cells were cotransfected with pmirGLO vectors containing either the wild-type (WT) or mutant (MUT) target sites plus either miR-451 mimics or the negative control. f, g Western blotting analysis of the expression of XPO-1 in LX-2 cells treated with XPO-1 inhibitor, verdinexor. h The expression of pri-miR-185, pre-miR-185 and miR-185 was determined by RT-qPCR in LX-2 cells treated with verdinexor. Data are shown as the means ± SEM obtained from triplicate experiments (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, n.s. nonsignificant).

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