Fig. 4: RUNX1-IT1 functions via the transcription factor RUNX1 in PC.

a FISH analysis showing the subcellular localization of RUNX1-IT1 in PANC-1 and SW1990 cells. b Histogram showing the expression level of RUNX1-IT1 in the subcellular fractions of PANC-1 and SW1990 cells, as analyzed by qPCR. c The interaction profile, which represents the protein interaction score (Y axis) relative to the RUNX1-IT1 RNA sequence (X axis), provides information about the region most likely to be bound by the protein. d The interaction matrix, which shows a heatmap of the RUNX1 protein (Y axis) and RUNX1-IT1 RNA (X axis) regions. The red shading in the heatmap indicates the interaction score of a single amino acid and nucleotide pair. e, f RIP assays were performed to validate RUNX1-IT1 binding to RUNX1 in PANC-1 and SW1990 cells transfected with RUNX1-IT1 overexpression or control vectors. g The chart shows information about the WT and mutant RUNX1-IT1 vectors. h RIP assays were performed to validate RUNX1-IT1 binding to RUNX1 in PANC-1 cells transfected with RUNX1-IT1 (WT) and mutant RUNX1-IT1 overexpression vectors. i EdU assays were used to assess proliferation in the three groups of PANC-1 cells. j The migration and invasion abilities of the three groups of cells were assessed by a transwell assay. k Histogram showing the proliferation rates of cotransfected cells in the three groups. l, m Histogram showing the number of migrated and invaded cotransfected cells in the three groups. n Representative MRI images of liver metastatic tumors in the three groups of mice are shown. Images of liver surfaces and HE staining of metastatic tumor lesions in the different groups are shown (scale bars, 1000 and 100 μm). (o) Histogram indicating the numbers of metastasized lesions in the three groups of mice (*P < 0.05, **P < 0.01, ***P < 0.001).