Fig. 3: Miconazole reduces neuroinflammation in LPS-treated mice brain. | Cell Death & Disease

Fig. 3: Miconazole reduces neuroinflammation in LPS-treated mice brain.

From: Antifungal drug miconazole ameliorated memory deficits in a mouse model of LPS-induced memory loss through targeting iNOS

Fig. 3

The expression levels of GFAP, Iba-1, and COX-2 in the mice brains were detected by western blotting using specific antibodies. The experiment was performed in triplicate. β-actin levels were measured to confirm equal protein loading. The values on the western blot bands and graph represent the arbitrary density measured by ImageJ (a). The mRNA levels of TNF-α (b), IL-1β (c), and IL-6 (d) were detected by qRT-PCR in mice hippocampus. The protein levels of TNF-α (e), IL-1β (f), and IL-6 (g) were detected by ELISA in mice hippocampus. Data are expressed as the mean ± SEM (t-test, #p < 0.05, ##p < 0.01, ###p < 0.0001 vs. Con, $p < 0.05, $$p < 0.01, $$$p < 0.0001 vs. MCZ, *p < 0.05, **p < 0.01, ***p < 0.0001 vs. MCZ + LPS).

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