Fig. 5: Miconazole effects on pro-inflammatory cytokine production in LPS-treated microglial BV2 cells and cultured astrocytes. | Cell Death & Disease

Fig. 5: Miconazole effects on pro-inflammatory cytokine production in LPS-treated microglial BV2 cells and cultured astrocytes.

From: Antifungal drug miconazole ameliorated memory deficits in a mouse model of LPS-induced memory loss through targeting iNOS

Fig. 5

The mRNA levels of pro-inflammatory cytokine were measured in 24-h LPS-treated (1 μg/mL) microglial BV2 cells and cultured astrocytes pretreated with MCZ (1.25, 2.5, 5, and 10 μM) for 1 h. The mRNA levels of TNF-α (a, b), IL-1β (c, d), and IL-6 (e, f) were detected by qRT-PCR in microglial BV2 cells (a, c, e) and cultured astrocytes (b, d, f). GAPDH for internal control. All experiments were performed three times with duplicate. Significant difference from the control group (t-test, p < 0.05). #Significant difference from the LPS-treated group (p < 0.05). *Significant difference from the MCZ-treated group (p < 0.05).

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