Fig. 7: Miconazole inhibits iNOS expression in in vivo and in vitro. | Cell Death & Disease

Fig. 7: Miconazole inhibits iNOS expression in in vivo and in vitro.

From: Antifungal drug miconazole ameliorated memory deficits in a mouse model of LPS-induced memory loss through targeting iNOS

Fig. 7

The mRNA levels of iNOS were measured in mice hippocampus tissues (a) and 24-h LPS-treated (1 μg/mL) cultured astrocytes (c) and microglial BV2 cells (d) pretreated with MCZ (1.25, 2.5, 5, and 10 μM) for 1 h. iNOS proteins were detected by western blotting using specific antibodies in mice hippocampus tissues (b), cultured astrocytes (e), and microglial BV2 cells (f). β-actin levels were measured to confirm equal protein loading. All experiments were performed three times with duplicate. The values on the western blot bands represent the arbitrary density measured by ImageJ. Data are expressed as the mean ± SEM (t-test, #p < 0.05 vs. Con, $p < 0.05 vs. MCZ, *p < 0.05 vs. MCZ + LPS). Significant difference from the control group (t-test, p < 0.05). #Significant difference from the LPS-treated group (p < 0.05). *Significant difference from the MCZ-treated group (p < 0.05).

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