Fig. 1: miR-130b-3p can be transferred to tumor cells through exosomes.
From: Exosomal miR-130b-3p targets SIK1 to inhibit medulloblastoma tumorigenesis
a The exosomes was assessed using transmission electron microscopy. Scale bar, 100 nm. b Western blot analysis of the exosomal marker, CD9 and CD63; Golgi marker, GM130. c Exosomal miR-130b-3p expression in healthy and MB patient plasma detected by RT-qPCR. d The expression of miR-130b-3p in healthy and MB patient PBMCs detected by RT-qPCR. e Exosomes were isolated from MB patient plasma, dyed with PKH67 (green) and cocultured with Daoy cells for 12 h, then dyed with Hoechst33258 (blue) and viewed with confocal microscopy (original magnifications ×200). f The expression of miR-130b-3p in Daoy cells cocultured with the exosomes isolated from MB patient plasma, detected by RT-qPCR. Data represent means ± SEMs from three independent experiments. *p < 0.05, ***p < 0.001.
