Fig. 2: Exosomal miR-130b-3p inhibit MB cell proliferation in vitro.
From: Exosomal miR-130b-3p targets SIK1 to inhibit medulloblastoma tumorigenesis
a, b RT-qPCR assay was used to detect the expression of miR-130b-3p in Daoy and D283 cocultured with the different concentrations of exosomes (10, 20, 50 μg/mL) collected from MB patient plasma. c, d The proliferative ability of Daoy and D283 treated with the different concentrations of exosomes derived from MB patient plasma by CCK-8. e Exosomes were isolated from culture supernatant from THP-1, dyed with PKH67 (green) and cocultured with Daoy, then dyed with Hoechst33258 (blue) and viewed with confocal microscopy (original magnifications ×200). f The expression of miR-130b-3p in Daoy cocultured with the exosomes collected from THP-1 culture supernatant, detected by RT-qPCR. The proliferative ability of Daoy (g) and D283 (h) treated with the exosomes derived from supernatant of THP-1 transfected with miR-130b-3p mimic or NC was tested by CCK-8. Cell proliferation of Daoy (i) and D283 (j) treated with supernatant of THP-1 transfected with miR-130b-3p mimic or NC and GW4869 was assessed by CCK-8. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01.
