Fig. 7: miR-130b-3p suppresses and SIK1 promotes MB tumorigenesis in vivo.
From: Exosomal miR-130b-3p targets SIK1 to inhibit medulloblastoma tumorigenesis
a Nude mice were injected with subcutaneous xenografts of Daoy cells overexpressing miR-130b-3p or NC and were euthanized after 8 weeks (five mice per group). b Tumor volume was recorded weekly after an injection of Daoy cells transfected with LV16-miR-130b-3p and LV16-NC. c Tumor weight was measured 8 weeks post injection with Daoy cells transfected with LV16-miR-130b-3p and LV16-NC. d, e miR-130b-3p expression was measured in mice tumors by RT-qPCR and FISH. f Mouse tumors were imaged 8 weeks after injection with Daoy cells, and demonstrated decreasing SIK1 expression (eight mice per group). g Tumor volume was recorded weekly after injection with Daoy cells transfected with sh-SIK1 and sh-GFP. h Tumor weight was measured 8 weeks post injection with Daoy cells transfected with sh-SIK1 or sh-GFP. i, j Hematoxylin and eosin (HE) staining (original magnifications ×200) of tumors and SIK1 and Ki67 expression in the tumor tissue samples, as determined through IHC. Data represent means ± SEMs from three independent experiments. *p < 0.05, **p < 0.01.
