Fig. 5: IL-8 promotes HNSCC progression through the STAT3 pathway. | Cell Death & Disease

Fig. 5: IL-8 promotes HNSCC progression through the STAT3 pathway.

From: The interaction of interleukin-8 and PTEN inactivation promotes the malignant progression of head and neck squamous cell carcinoma via the STAT3 pathway

Fig. 5

a The expression of STAT3, p-STAT3, PTEN, p-PTEN, and snail was detected by western blot after 100 ng/ml IL-8 treatment. b STAT3, p-STAT3, PTEN, p-PTEN, and snail expression were determined by western blot after 100 ng/ml IL-8 and 50 μg/ml Reparixin treatment. c, d Migration ability was examined by a transwell assay and a wound healing assay after stimulation with 100 ng/ml IL-8, 10 μM crypto, 100 ng/ml IL-8 + 10 μM crypto, or blank medium. Scale bar, 250 μm (transwell assay) and 100 μm (wound healing assay). e The CCK8 assay was applied to detect proliferation ability. f The expression of MMP9, MMP2, vimentin, STAT3, p-STAT3, and snail in each group was detected by western blot; *P < 0.05, **P < 0.01.

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