Fig. 6: HOXA5 inhibited the expression of cyclinD1 via suppressing the Wnt/β-catenin pathway in cervical cancer cells. | Cell Death & Disease

Fig. 6: HOXA5 inhibited the expression of cyclinD1 via suppressing the Wnt/β-catenin pathway in cervical cancer cells.

From: HOXA5 inhibits the proliferation and neoplasia of cervical cancer cells via downregulating the activity of the Wnt/β-catenin pathway and transactivating TP53

Fig. 6

a Heatmap of known genes for Wnt/β-catenin pathway in SiHa-GFP cells (left) and SiHa-HOXA5 cells (right) using data from RNA-seq. Data were log2 normalized. b A gene set enrichment (GSEA) assay using data from RNA-seq. c–f TOP/FOP-Flash reporter assays were carried out in HOXA5-modified cervical cancer cells. g–j Real-time PCR analysis was shown for the mRNA levels of Wnt/β-catenin pathway key genes in HOXA5-modified cervical cancer cells. k The expression of Wnt/β-catenin pathway key proteins in HOXA5-modified cervical cancer cells was determined by western blot. The nuclear β-catenin of HOXA5-modified cervical cancer cell was determined by western blot (l) and immunocytochemistry (m). Data represent mean ± SD of triplicate experiments and statistical analysis was done with Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

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