Fig. 7: HOXA5 regulates the expression of TP53 in cervical cancer cells. | Cell Death & Disease

Fig. 7: HOXA5 regulates the expression of TP53 in cervical cancer cells.

From: HOXA5 inhibits the proliferation and neoplasia of cervical cancer cells via downregulating the activity of the Wnt/β-catenin pathway and transactivating TP53

Fig. 7

The expression of TP53 in HOXA5-modified cervical cancer cells was determined by real-time PCR (a) and western blot (b). The expression of p53 in xenograft was determined by IHC (c). d–f The expression of HOXA5, p53, and p21 in clinical cervical cancer specimens was determined by western blot and the correlation was analyzed. g HOXA5 expression was significantly positively correlated with the expression of p53 (r = 0.4118, p < 0.001) in cervical cancers (data from GEO database). h, i The TP53 promoter structure was constructed and luciferase activity relative to Renilla control was measured in HeLa-HOXA5 and SiHa-HOXA5 cells. j The qChIP assay is shown in HeLa-HOXA5 and SiHa-HOXA5 cells immunoprecipitation by HOXA5 antibody and IgG antibody (as the negative control). k An experimentally defined transcription factor binding sites of HOXA5 was found in the JASPAR CORE database. The data were shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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