Fig. 3: TTP and HuR modulated mRNA and protein levels of IL-17 and claudin-1 in cultured mouse podocytes. | Cell Death & Disease

Fig. 3: TTP and HuR modulated mRNA and protein levels of IL-17 and claudin-1 in cultured mouse podocytes.

From: RNA-binding proteins tristetraprolin and human antigen R are novel modulators of podocyte injury in diabetic kidney disease

Fig. 3

a RNA immunoprecipitation in podocyte extracts using anti-HuR or anti-TTP antibodies (top). Total RNA was extracted from the immunoprecipitated complexes, reverse transcribed, and analyzed by PCR using primers specific to claudin-1 and IL-17 3′-UTRs (bottom). b, c Podocytes treated with normal-glucose solution (5.6 mM) were transfected with TTP-specific siRNA (TTP siRNA) or scrambled siRNA (sc-siRNA 1) or with HuR-specific siRNA (HuR siRNA) or scrambled siRNA (sc-siRNA 2). IL-17 and claudin-1 mRNA levels were determined by qRT-PCR (b) and protein levels by immunoblotting (c). Data are expressed as the mean ± SD; n = 6 for each separate experiment. *p < 0.05 vs. control podocytes treated only with a normal-glucose solution (CON).

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