Fig. 4: PRR14 activates the PI3K signal pathway. | Cell Death & Disease

Fig. 4: PRR14 activates the PI3K signal pathway.

From: Oncogene PRR14 promotes breast cancer through activation of PI3K signal pathway and inhibition of CHEK2 pathway

Fig. 4

Venn diagram presenting comparative analysis of DE genes between high- and low- PRR14-expressing Basal, LumA and LumB cases deposited in the TCGA database. The cutoff of P < 0.001 is considered significant to determinate DE transcripts (a). Heatmap presenting 273 common DE genes in Basal, Her2, LumA and LumB subtypes of breast cancer (b). The gene expression levels are normalized and log2-transformed, reaching from −2 to +2 that corresponds to blue and red colors on heatmap, respectively. P-value and over-represented pathways from 273 common DE genes are analyzed using ConsensusPathDB (c). Key components of the PI3K/Akt/mTOR signaling pathway are detected by immunostaining in both MCF7 and MDA-MB-231 cell lines transfected with siRNA to reduce PRR14 expression (d), as well as established PRR14-overexpressing and its control cell lines (e). Serum is added to the medium for indicated time after 12-h-serum starvation to stimulate the signaling pathway in PRR14-overexpressing and the control cell lines.

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