Table 2 Antibody dilutions for Western blot.

From: Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB

Antibody name

Dilution

Akt1, mTOR

1/5000

p-Akt1, p-p65, p-IκBα, IκBα, and p-mTOR

1/2500

p52, β-actin

1/2000

S6K

1/1500

CRYAB, NIK, p65, IKKα, p-IKKα, p-IKKβ, IKKβ,

 

IKK, p-IKK, IL-1β, Akt2, p-Akt2, Arg-1, and p-4E-BP

1/1000

p-S6K

1/800

4E-BP

1/500

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