Fig. 5: Endothelium generation of breast cancer cells induced by Snail depends on VEGF signaling.
From: Snail promotes the generation of vascular endothelium by breast cancer cells
a Representative FACS of MCF-7 cells stably expressing EV or Snail treated with or without VEGF-neutralized antibody or VEGFR2 inhibitor using CD31-FITC antibodies. Graphs show percentage of CD31-positive cells. Results shown are mean ± SD of three independent experiments. b IF analysis of MCF-7 cells stably expressing EV or Snail treated with or without VEGF neutralized antibody or VEGFR2 inhibitor. Graphs show percentage of CD31-positive or VWF-positive cells. Results shown are mean ± SD of three independent experiments. Scale bar, 20 μm. c Lineage tracing of MCF-7 cells stably expressing EV or Snail with CD31pro-GFP, CD105pro-GFP, and CD144pro-GFP treated with or without VEGF-neutralized antibody or VEGFR2 inhibitor. Scale bar, 10 μm. Graphs show percentage of GFP-positive cells. Results shown are mean ± SD of three independent experiments. d Tube-forming assays and DiI-AcLDL uptake assays with copGFP-expressing or Snail-copGFP-expressing MCF-7 cells treated with or without VEGF-neutralized antibody or VEGFR2 inhibitor. Statistical analysis of tube length is shown in the right panel. Results shown are mean ± SD of three independent experiments. Scale bar, 100 μm. Red: DiI-AcLDL staining. *P < 0.05, **P < 0.01.
