Fig. 4: NOD2 signalling. | Cell Death & Disease

Fig. 4: NOD2 signalling.

From: Novel XIAP mutation causing enhanced spontaneous apoptosis and disturbed NOD2 signalling in a patient with atypical adult-onset Crohn’s disease

Fig. 4

Patient and control (n = 5) peripheral blood was stimulated with MDP (10 µg/ml) for 20 min, and phosphorylation of a MAP kinases were detected by phospho-flow cytometry b and by Western blot. NFκB signalling, expressed as IκB degradation and NFκB phosphorylation, was detected by c flow cytometry and d by Western blot after MDP (10 µg/ml) stimulation of patient and control (n = 5) peripheral blood cells. e Production of IL-1β, TNFα, IL-6 and IL-12p70 after MDP (10 µg/ml) or LPS (1 µg/ml) stimulation of patient and control (n = 5) PBMCs was detected by Luminex.

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