Fig. 3: ΔNp63α promotes SNAI1 expression and EEC–EMT in vitro.
From: ΔNp63α-induced DUSP4/GSK3β/SNAI1 pathway in epithelial cells drives endometrial fibrosis
a Protein levels of SNAI 1 were detected by western blotting in EECs after incubation with Ad-ΔNp63α or Ad-CTL for 48 h (n = 3). Relative band intensities were analyzed with Image J. b Representative images of SNAI1 immunostaining in normal (n = 30) and fibrotic endometria (n = 30). Scale bars, 50 μm. c Protein levels of SNAI 1 were detected by western blotting in endometria from normal control (n = 5) and IUA patients (n = 5). Relative band intensities were analyzed with ImageJ. d Representative images of ΔNp63α, E-cadherin, N-cadherin, and α-SMA immunofluorescence staining in EECs after incubation with Ad-ΔNp63α or Ad-CTL for 48 h (n = 3). Scale bars, 20 μm. e Protein levels of E-cadherin, N-cadherin, α-SMA, and β-actin were detected by western blotting in EECs after incubation with Ad-ΔNp63α or Ad-CTL for 48 h (n = 3). Relative band intensities were analyzed with ImageJ. Error bars, mean ± SD; **p < 0.01, and ***p < 0.001.
