Fig. 4: ΔNp63α induces the downregulation of the SNAI1 inhibitor, GSK3β in EECs.
From: ΔNp63α-induced DUSP4/GSK3β/SNAI1 pathway in epithelial cells drives endometrial fibrosis
a Immunoblotting of pGSK3β(S9), GSK3β, and β-actin in EECs after incubation with Ad-ΔNp63α or Ad-CTL for 48 h (n = 3). Relative band intensities were analyzed with Image J. b Immunoblotting of SNAI 1, GSK3β, and β-actin in EECs after incubation with Ad-ΔNp63α or Ad-CTL and TDZD8 or TWS119 for 48 h (n = 3). Relative band intensities were analyzed with ImageJ. c Representative images of GSK3β immunostaining staining in normal (n = 30) and fibrotic endometria (n = 30). Scale bars, 10 μm. d Protein levels of pGSK3β(S9) and GSK3β were detected by western blotting in endometria from normal control (n = 5) and IUA patients (n = 5). Error bars, mean ± SD.; *p < 0.05, **p < 0.01, and ***p < 0.001.
