Fig. 5: ΔNp63α induced DUSP4 downregulates GSK3β expression in EECs.
From: ΔNp63α-induced DUSP4/GSK3β/SNAI1 pathway in epithelial cells drives endometrial fibrosis
a Protein level of DUSP4 was detected by western blotting in EECs after co-incubation with Ad-ΔNp63α or Ad-CTL for 48 h (n = 3). Relative band intensities were analyzed with Image J. b The fluorescence activity of DUSP4 promoter containing plasmid which was transfected into EECs with Ad-CTL or Ad-ΔNp63α for 12 h (n = 6). c The correlation of DUSP4 and GSK3β expression in endometria of IUAs (n = 21). Spearman’s correlation coefficient R = 0.4138, p < 0.05. d The correlation of DUSP4 and SNAI1 expression in endometria of IUAs (n = 21). Spearman’s correlation coefficient R = 0.5338, p < 0.05. e Immunoblotting of DUSP4, pGSK3β(S9), GSK3β, SNAI1, and β-actin in EECs after transfection with pCDNA3.1-DUSP4 or pCDNA3.1 for 24 h (n = 3). Relative band intensities were analyzed with ImageJ. f Immunoblotting of DUSP4, pGSK3β(S9), GSK3β, SNAI1 and β-actin in EECs after transfection with DUSP4-specific siRNAs for 24 h (n = 3). Relative intensities of bands were analyzed with Image J. g Immunoblotting of DUSP4 and β-actin in endometrium from IUA patients (n = 5) and normal control (n = 5). Relative band intensities were analyzed with ImageJ. Error bars, mean ± SD; *p < 0.05, **p < 0.01, and ***p < 0.001.
