Fig. 1: (+)JQ1 reduced PCN-mediated macrophage death. | Cell Death & Disease

Fig. 1: (+)JQ1 reduced PCN-mediated macrophage death.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 1: (+)JQ1 reduced PCN-mediated macrophage death.

Cells were pre-treated with (+)JQ1 or (−)JQ1 24 h before experiment, unless otherwise specified. CCK8 assay showing the cell viability of a RAW and b AMs at 24 h post-PCN challenge. c Flow cytometry analysis and d quantified apoptosis rates of double-stained RAW cells at 8 h post-PCN challenge. AnnexinV-488A (apoptotic fraction: Q2 and Q3 quadrants) and PI (necrotic fraction: Q1 quadrant). Western blot showing the expression of p53 and Bcl-2 in RAW (g) and AMs (e), respectively, as well as cleaved-caspase3 and total caspase 3 at 8 h post-PCN challenge (i). f, h Densitometry analysis from e, g. j Ratio between cleaved-caspase 3 and total caspase 3 from (i). (Data shown were mean ± SD from three independent experiments. *P < 0.05, **P < 0.01compared with indicated group, ns non-significant. One-way ANOVA test followed by t-test).

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