Fig. 2: (+)JQ1 regulated oxidant and anti-oxidant pathways in PCN-challenged macrophages. | Cell Death & Disease

Fig. 2: (+)JQ1 regulated oxidant and anti-oxidant pathways in PCN-challenged macrophages.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 2: (+)JQ1 regulated oxidant and anti-oxidant pathways in PCN-challenged macrophages.The alternative text for this image may have been generated using AI.

a Representative images showing DHE-stained intracellular ROS in RAW cells at 3 h post-PCN challenge. b CCK8 assay showing the viability of RAW cells at 24 h post-PCN challenge. c RT-qPCR showing the mRNA expression levels of indicated genes in RAW cells at 8 h post-PCN challenge. (*P < 0.05, **P < 0.01 compared with indicated group. Bar graphs represent three independent experiments, each performed in triplicates). d, f Western blot showing the expression of Nrf2 and Keap1 in RAW cells and AMs at 8 h post-PCN challenge. e, g Densitometry analysis of expression ratio between Nrf2 and Keap1 in d, f. h Western blot showing the cytosolic and nuclear distribution of Nrf2 and p65 in RAW cells. (Data shown were the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 compared with indicated group. One-way ANOVA test followed by t-test).

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