Fig. 3: (+)JQ1 repressed PCN-induced RNS production. | Cell Death & Disease

Fig. 3: (+)JQ1 repressed PCN-induced RNS production.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 3: (+)JQ1 repressed PCN-induced RNS production.

NO production was measured in the medium of a RAW cells and b AMs at 8 h PCN-post challenge. c CCK8 assay showing the cell viability of RAW cells with the independent or combined treatments of indicated reagents for 24 h. d LDH release and e p53 expression of RAW cells at 8 h post-treatment of independent or combined use of indicated reagents. f Concentration of ONOO− measured in RAW cell medium at 8 h post-treatment of the indicated reagents. g, h Relative induction of ONOO− in RAW cells at 8 h post-treatment of the indicated reagents with or without pre-treatment of (+)JQ1. (Data shown were the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001compared with indicated group. ns non-significant. One-way ANOVA test followed by t-test).

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