Fig. 4: shRNA-mediated NOS2 silencing reduced PCN-mediated RNS production and macrophage death. | Cell Death & Disease

Fig. 4: shRNA-mediated NOS2 silencing reduced PCN-mediated RNS production and macrophage death.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 4: shRNA-mediated NOS2 silencing reduced PCN-mediated RNS production and macrophage death.

a RT-qPCR showing the relative mRNA levels of NOS2 in RAW cells at indicated time points post-PCN treatment (100 μM). b Western blotting showing iNOS expression in RAW cells transfected with different shRNA with or without PCN treatment for 8 h. A non-target scrambled oligonucleotide sequence functioned as the negative control (sh#NC). c NO production measured in cell medium under the same condition in b. d, e Cell viability of NOS2 knock-down RAW cells treated with PCN or combination of indicated reagents for 24 h. (Data shown were the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, compared with indicated group, ns non-significant. One-way ANOVA test followed by t-test).

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