Fig. 6: BRD4 knock-down increased macrophage survival and interrupted RNS production upon PCN stress. | Cell Death & Disease

Fig. 6: BRD4 knock-down increased macrophage survival and interrupted RNS production upon PCN stress.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 6: BRD4 knock-down increased macrophage survival and interrupted RNS production upon PCN stress.

a The mRNA and b protein levels of Brd4 in RAW cells stably expressing individual shRNA sequences. c Cell viability of BRD4 knock-down cells (sh#475 and sh#1648) at 24 h post-treatment of PCN. d Western blot showing levels of Brd4, iNOS and p53 in BRD4 knock-down cells at 8 h post-treatment of PCN. e The relative production of ONOO- induced in BRD4 knock-down cells at 24 h post-treatment of PCN. f Cell viability of BRD4 knock-down cells treated with independent or combined use of indicated reagents. A non-target scrambled oligonucleotide sequence functioned as the negative control (sh#NC). g The schematic diagram depicting the proposed mechanisms by which (+)JQ1 protects PCN-mediated macrophage cell death (see Discussion). (Data shown were the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, compared with indicated group. ns non-significant. One-way ANOVA test followed by t-test).

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