Fig. 7: Effects of (+)JQ1 on macrophage activities and PA-mediated mice lung infection. | Cell Death & Disease

Fig. 7: Effects of (+)JQ1 on macrophage activities and PA-mediated mice lung infection.

From: Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways

Fig. 7: Effects of (+)JQ1 on macrophage activities and PA-mediated mice lung infection.

a Fluorescence microscopy showing phagocytosis of FITC-labeled heat-inactivated PA (MOI 300:1) in PCN-stressed BMDMs with or without pre-treatment of (+)JQ1. b Flow cytometry quantification of engulfed FITC fluorescence in BMDMs shown in a. c RT-qPCR showing the relative expression of pro-inflammation genes in BMDMs at 12 h post-PCN challenge with or without pre-treatment of (+)JQ1. d The schematic diagram showing the experiment strategy of animal experiments: C57BL/6 mice were consecutively given intra-peritoneal injection of 50 mg/kg (+)JQ1 or 5% DMSO as control for 5 days (−4d to 0d). The intra-tracheal injection of 1.0 × 107 cfu PA with or without 50 μM PCN were given at 0d. e Bacteria counts in lung homogenates (Data were shown as means ± SD of cfu per gram of lung tissue from five mice in each group). f H&E staining showing histology change of infected lung (×10). (Data shown were the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, compared with indicated group, One-way ANOVA test followed by t-test).

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