Fig. 3: The binding of NORAD and FUBP1 is essential for NORAD to induce apoptosis. | Cell Death & Disease

Fig. 3: The binding of NORAD and FUBP1 is essential for NORAD to induce apoptosis.

From: NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis

Fig. 3: The binding of NORAD and FUBP1 is essential for NORAD to induce apoptosis.The alternative text for this image may have been generated using AI.

a qRT-PCR analysis for the binding of NORAD, SNHG1, and GAPDH expression by an anti-FUBP1 antibody compared with that by an IgG control antibody in 293FT cells. SNHG1 and GAPDH served as the positive and negative controls, respectively. b qRT-PCR analysis for the binding of NORAD on FUBP1 in EC cells with Aza treatment by RIP assays. c Western blot for the FUBP1 protein (lower panel) pulled down by truncated NORAD (NORAD-1, -2, -3, and -4; upper panel). d The expression levels of full-length NORAD and fragments transfected in ISK and SPEC-2 cells were detected by qRT-PCR. e Cell-counting assays for ISK and SPEC-2 cells transfected with full-length NORAD and fragments, respectively. f The percentage of apoptotic cells transfected with full-length NORAD and fragments, evaluated by FACS analysis. g Western blot for the expression of cleaved PARP and cleaved caspase-3 after ectopic expression of full-length NORAD and fragments. The results were determined from triplicates, and the error bars represented as the mean ± SD, */# P < 0.05, **P < 0.01, ***P < 0.001. FUBP1 far upstream element-binding protein 1, SNHG1 small nucleolar RNA host gene 1, RIP RNA immunoprecipitation, YFP yellow fluorescent protein.

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