Fig. 2: Knockdown of circ_0111277 promotes trophoblast cell migration and invasion.

Migration (a) and invasion (b) capacities of HTR-8/SVneo or JEG-3 cells with or without transient transfection of si-circ_0111277. Nonsense siRNA served as a negative control (si-NC). c TIMP1/2 protein levels in HTR-8/SVneo and JEG-3 cells transfected with si-circ_0111277 were determined by western blot assays. d TIMP1/2 mRNA levels in HTR-8/SVneo and JEG-3 cells transfected with si-circ_0111277 were measured by qPCR analysis. e The concentrations of PLGF, β-hCG, and TNF-α in the supernatants of HTR8/SVneo and JEG-3 cells treated with si-circ_0111277, as determined by ELISA.