Fig. 5: hsa_circ_0111277 regulates the Notch signal pathway through the miR-494-3p/HTAR1 axis. | Cell Death & Disease

Fig. 5: hsa_circ_0111277 regulates the Notch signal pathway through the miR-494-3p/HTAR1 axis.

From: Circular RNA circ_0111277 attenuates human trophoblast cell invasion and migration by regulating miR-494/HTRA1/Notch-1 signal pathway in pre-eclampsia

Fig. 5

The Notch-1 protein levels between PE placentas (n = 3) and normal pregnancy placentas (n = 3) were mirrored, as revealed by western blot assays. a Notch-1 protein levels in HTR8/SVneo and JEG-3 cells after si-circ_0111277-modulated silencing of hsa_circ_0111277 were measured by western blot assays. b VEGFR3 mRNA levels in HTR8/SVneo and JEG-3 cells transfected with si-circ_0111277 were monitored by qPCR. c Notch-1 protein d and VEGFR3 mRNA levels e in HTR8/SVneo and JEG-3 cells upon miR-494 inhibition treatment were measured by western blot and qPCR assays, respectively. f Notch-1 protein in HTR8/SVneo and JEG-3 cells co-transfected with si-circ_0111277 or si-circ_0111277+pLV3-HTRA1 was detected by western blot assay.

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