Fig. 3: Abnormal spindle in Hfm1 knockout oocytes.
From: Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis
a The micrographs of oocytes from Control and Hfm1-cKO females (3-week-old) after IVM. White arrows point to no first polar body (PB1) extrusion oocytes. b The PB1 extrusion rate of oocytes from Control and Hfm1-cKO females (3-week-old) by IVM (n = 4). c Percentage of in vivo matured MII oocytes with abnormal spindle from Control and Hfm1-cKO females (3-week-old) (n = 3). d Percentage of in vivo matured MII oocytes with no actin cap from Control and Hfm1-cKO females (3-week-old) (n = 3). e Representative spindles from MII oocytes ovulated by Control and Hfm1-cKO females (3-week-old) in vivo. (A) Normal MII stage. (B) abnormal MII with a spindle and a decreased actin cap fluorescence intensity. (C) Abnormal MII with no spindle and no actin cap. (D) Abnormal MII with incomplete division spindle and no actin cap. Microtubules, Chromosomes, and F-actin are stained green, blue, and red, respectively. Yellow arrows pointed to actin cap. f Profiles of phalloidin fluorescence intensity along the yellow line in oocytes (A) and (B). The yellow line perpendicular to the length axis of the spindle g Quantitative analysis of peak actin immunofluorescence intensities in profiles of Control and Hfm1-cKO oocytes with actin cap (n = 10). *P < 0.05. **P < 0.01. ***P < 0.001. Significance was determined by two-tailed Student’s t tests. Data represent the mean ± SEM.
