Fig. 5: Disrupted MAPK pathway in Hfm1 knockout oocytes.
From: Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis
a Denuded oocytes cultured for 8 h (MI) and stained with p-Mapk (red), β-Tubulin (green), and DAPI (blue). White arrows point to p-Mapk signals in oocytes. b Western blot analysis of Erk1/2 expression in Control and Hfm1-cKO ovaries (n = 5). c Western blot analysis of p-Mapk expression in Control and Hfm1-cKO ovaries (n = 5). d Western blot analysis of p-Mapk in Control and Hfm1-cKO oocytes at MI stage (n = 4). Protein lysates from 100 oocytes were loaded in each lane. **P < 0.01. ***P < 0.001. Significance was determined by two-tailed Student’s t tests. Data represent the mean ± SEM.
