Fig. 6: CircAGFG1 regulated YY1 expression through absorbing miR-4262 and miR-185-5p.
From: CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1
a Venn diagram showed 31 miRNAs which could bind to both circAGFG1 and YY1 3′-UTR. b RNA pull-down assay was carried out to detect the binding capacity between circAGFG1 and miRNAs. c Luciferase reporter assay was performed to verify the interaction between circAGFG1 and miR-4262 or miR-185-5p. d Luciferase reporter assay was performed to verify the interaction between YY1 3′-UTR and miR-4262 or miR-185-5p, as well as the competitive effect of circAGFG1. e RIP assay was carried out to further confirm the enrichments of YY1, miR-4262, miR-185-5p and circAGFG1 in IgG group and Ago2 group. We repeated the experiments three times to ensure the accuracy of the experiments. **P < 0.01.
