Fig. 3: Time-lapse tracking of BMSCs co-cultured with atRA-treated NTD embryos. | Cell Death & Disease

Fig. 3: Time-lapse tracking of BMSCs co-cultured with atRA-treated NTD embryos.

From: Transamniotic mesenchymal stem cell therapy for neural tube defects preserves neural function through lesion-specific engraftment and regeneration

Fig. 3: Time-lapse tracking of BMSCs co-cultured with atRA-treated NTD embryos.

a Representative time-lapse live cell images of BMSC migration. The white dotted line demarcates the edge of the embryo. Red hollow crosses indicate that the BMSCs migrated spontaneously into the neural tube. The numbers indicate the 35 cells used for cell tracing analysis. b The tracing analysis cell movement for 35 cells monitored from 0 to 16 h. The small circles represent the starting positions of the cells, and the arrows indicate the direction and distance traveled by the cells. c Histogram of cell numbers of three migration patterns. d The distance between the BMSCs and the embryo versus culture time. The median distance at each time point is indicated by the short black line. * indicate significant difference compared with 0 h, † indicate significant difference compared with 6 h, ‡ indicate significant difference compared with 12 h, P < 0.05. e The migrating BMSCs (red arrowhead) transformed into mature neuron-like cells with long axons that made contact with other BMSCs (white arrowheads). Scale bars: 200 μm.

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