Fig. 6: In utero transamniotic transplanted BMSCs differentiate into multi-lineage cells. | Cell Death & Disease

Fig. 6: In utero transamniotic transplanted BMSCs differentiate into multi-lineage cells.

From: Transamniotic mesenchymal stem cell therapy for neural tube defects preserves neural function through lesion-specific engraftment and regeneration

Fig. 6: In utero transamniotic transplanted BMSCs differentiate into multi-lineage cells.

a Immunofluorescence staining for K19 (red) in cultured BMSCs. b Percentage of K19+ BMSCs in vitro and in the abnormal skin region of fetuses. *Significant difference compare to BMSCs cultured in vitro group, P < 0.05. c Dual-labeling for K19 (red) and GFP (green) in the tissue sections of the defective spinal cord after BMSC transplantation. White arrows denote the localizations of representative double-positive cells. d–g Representative immunofluorescence images showing transplanted BMSCs expressing SYN (d), SYT (e), BRN3A (f), and ISLET1 (g) in the defective spinal cord. Scale bars: 50 μm. h Quantitative analysis of SYN, SYT, BRN3A, and ISLET1 in transplanted BMSCs in defective spinal cords. The percentage of BMSCs expressing these markers were determined as the number of double positive cells/total GFP positive cells (40× field, n = 10).

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