Fig. 5: Transcriptomic changes in pancreatic endocrine progenitors (EPs) derived from FOXA2+/− iPSCs.
From: Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency
A Volcano plot plotting the log2 fold change (FC) and the adjusted p-value for all the transcripts. B Pathway enrichment analysis on upregulated and downregulated differentially expressed genes (DEGs) in FOXA2+/− iPSC-derived EPs using DAVID function annotation tool. The enriched pathways were plotted against −log10 (p-value). C Heatmaps of pathway-associated DEGs in FOXA2+/− iPSC-derived EPs compared to those derived from Ctr-iPSCs. The downregulated genes were associated with pancreatic development, maturity-onset diabetes of the young (MODY), and NOTCH signaling pathways, while the upregulated genes were associated with the WNT signaling pathway and nervous system development. The relative value for each gene is depicted by color intensity, with red indicating upregulated and blue indicating downregulated genes. D RNA-Seq results were validated using qPCR for the upregulated genes associated with WNT signaling and nervous system development. Data are represented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001.
