Fig. 1: SNHG18 is transactivated by MKL1 in NSCLC cells. | Cell Death & Disease

Fig. 1: SNHG18 is transactivated by MKL1 in NSCLC cells.

From: MKL1-induced lncRNA SNHG18 drives the growth and metastasis of non-small cell lung cancer via the miR-211-5p/BRD4 axis

Fig. 1

A, B Quantitative PCR analysis of indicated lncRNAs in A A549 and B H1299 cells transfected with empty vector or MKL1-expressing vector. *P < 0.05 vs. vector, Student’s t-test. n = 3. C Quantitative PCR analysis of MKL1 mRNA in NSCLC cells transfected with negative control shRNA (shCtrl) or 2 different shRNAs targeting MKL1 (shMKL1#1 and shMKL1#2). *P < 0.05 vs. shCtr, one-way ANOVA followed by Bonferroni’s test. n = 3. D Quantitative PCR analysis of SNHG18 expression in MKL1 shRNA-transfected NSCLC cells. *P < 0.05 vs. shCtr, one-way ANOVA followed by Bonferroni’s test. n = 3. E A549 cells were transfected with SNHG18 promoter reporters together with MKL1-expressing vector, and luciferase activities were measured 24 h after transfection. *P < 0.05 vs. vector, Student’s t-test. n.s. indicates no significance relative to vector. n = 3. F ChIP assay showed the occupancy of MKL1 protein on the promoter of SNHG18 (−1269 to −1072 bp) but not GAPDH (−781 to −435 bp). *P < 0.05 vs. control IgG, Student’s t-test. n.s. indicates no significance relative to control IgG. n = 3.

Back to article page