Fig. 4: Fibrogenic differentiation of mdx FAP cell states.
From: SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors
a Experimental design to induce fibrogenic differentiation of mdx FAP cell states. GM = growth medium; FM = fibrogenic medium. b Bar plot presenting alpha-SMA-positive area per cell in each field (n = 6). Statistical significance was evaluated using Two-way ANOVA. c Representative microphotographs of (b). Immunofluorescence against alpha-SMA (yellow) and nuclei counterstaining (blue, Hoechst 33342). Scale bar 100 μm. d–f Bar plots showing Col3a1, Col1a1 (n = 3) and Timp1 (n = 4) mRNA expression. Following 5 days of differentiation samples were analysed by Real-Time PCR. Statistical significance was estimated through a Two-way ANOVA. g, h Bar plots showing Col3a1 and Col1a1 mRNA expression immediately after sorting (n = 3). Samples were analysed by Real-Time PCR using 2-ΔΔCt comparative method. Tubulin mRNA was set as reference gene. Statistical significance was evaluated by Student’s t test. Data are presented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
