Fig. 5: SCA1-High-FAPs have a transcriptional profile committed to the adipogenic differentiation.
From: SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors
a Multidimensional scaling (MDS) plot of FAP cell states transcriptome. b Volcano plot of log10(adjust p-value) versus log2(fold change) showing upregulated (in green) and downregulated (in purple) genes in SCA1-Low-FAPs. c Heatmap of the Difference of the different biological replicates (n = 4). d Profile plots of the Difference of the downregulated (upper panel, purple cluster in Fig. 5c) and upregulated genes (lower panel, orange cluster in Fig. 5c) in SCA1-Low-FAPs. The right panel shows the top 4 Biological processes GO terms significantly enriched (FDR < 0.05) for upregulated and downregulated genes in SCA1-Low-FAPs. e A network in which each node represents a gene transcriptionally regulated by PPAR-gamma (from TRRUST). Red edges: genes activated by PPAR-gamma; blue edges: genes repressed by PPAR-gamma; grey edges: unknown mechanism. The nodes are filled according to the Fold Change. f Bar plot showing the percentage of PPAR-gamma positive cells after 5 days in GM (n = 3). g Representative images of bar plot in the previous panel. PPAR-gamma in red and nuclei in blue. Scale bar 100 μm. Statistical analysis was performed with Student’s t test. Data are presented as mean ± SEM. *p ≤ 0.05.
