Fig. 3: CUX1 knockdown inhibited cell migration and invasion by regulating EMT progression and MMPs expression in vitro and in vivo.
From: Overexpressed P75CUX1 promotes EMT in glioma infiltration by activating β-catenin
a Representative images of intracranial tumor xenograft with U87 cell lines in nude mice. HE and IHC of human Nestin staining revealed that the CUX1 knockdown inhibited the aggressive phenotype in vivo (NC, n = 5; ShCUX1, n = 5). b WB assay revealed that the CUX1 knockdown in U87 and U251 cells regulated the expression of EMT-associated proteins and MMPs in vitro, including reduced the expression of N-Cadherin, Slug, Snail, β-Catenin, MMP2, and MMP9, and enhanced the expression of E-Cadherin, Claudin, and ZO-1(*P < 0.05). c IHC staining showed that CUX1 knockdown inhibited the EMT-related protein expression including N-Cadherin, Slug, β-catenin, MMP2, and MMP9 in vivo.
