Fig. 4: P75CUX1 regulated β-catenin expression and activated WNT/β-catenin signaling pathway.
From: Overexpressed P75CUX1 promotes EMT in glioma infiltration by activating β-catenin
a Gene set enrichment analysis (GSEA) showed that there was a significant correlation between CUX1 mRNA expression and the WNT/β-catenin pathway in glioma (data from TCGA, CGGA, and GSE4290 databases). b Spearman correlation analysis indicated a significant correlation between the mRNA expression of CUX1 and β-catenin in glioma and NB tissues (P < 0.001, R = 0.72, data from TCGA and GTX database). c IF assay revealed that CUX1 knockdown inhibited the total β-catenin expression and β-catenin accumulation in the nucleus of U87 and U251 cells. d WB showed that P-β-catenin (Ser33/37/Thr41) expression increased while P75CUX1 knockdown. e qRT-PCR analysis showed that the CUX1 knockdown reduced the mRNA expression of β-catenin in U87 and U251 cells (**P < 0.01). f TOP/FOP flash assay detected that the CUX1 knockdown suppressed the activity of Wnt/β-catenin in the nucleus. Bars indicated the relative luciferase activity (****P < 0.0001).
