Fig. 2: HELLS binds target gene promoters. | Cell Death & Disease

Fig. 2: HELLS binds target gene promoters.

From: The DNA-helicase HELLS drives ALK− ALCL proliferation by the transcriptional control of a cytokinesis-related program

Fig. 2: HELLS binds target gene promoters.

A qRT-PCR validation of significantly downregulated genes obtained from RNA-sequencing. White bars represent the most significantly downregulated genes (FDR < 0.1) and gray bars represent less significant downregulated genes (FDR > 0.1) in TLBR-2 HELLSKD cells and MAC2A HELLSKD cells after 48 h of doxycycline (DOX) induction. Each data represent mean ± SEM (n = 3). Two-tailed t-test. *p < 0.05; **p < 0.01. B qRT-PCR validation of significantly downregulated genes obtained from RNA-sequencing. White bars represent the most significantly downregulated genes (FDR < 0.1) and gray bars represent less significant downregulated genes (FDR > 0.1) in TLBR-2 BlackMambaKD cells and MAC2A BlackMambaKD cells after 6 days of doxycycline (DOX) induction. Each data represent mean ± SEM (n = 3). Two-tailed t-test. *p < 0.05; **p < 0.01. C ChIP qRT-PCR detection of HELLS antibody in a panel of target gene promoters in TLBR-2 and MAC2A.P4_BlackMamba and α-satellite were used as positive and negative controls, respectively. The values represent the relative fold enrichment over IgG and are indicated as mean ± SEM (n = 3). Two-tailed t-test. *p < 0.05; **p < 0.01.

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