Fig. 3: HELLS interacts with the transcriptional factor YY1.
A Schematic representation of CDC42SE2 genomic locus showing the position, the level of transcription and the enrichment of H3K4me3 on its putative promoter. B The Venn diagram represents the intersection between transcriptional factors (TFs) identified by JASPAR (n = 607) and PROMO (n = 26) TFs binding prediction tools. The intersection shows the number of HELLS-predicted and common TFs (n = 9). C JASPAR and PROMO similarity scores are represented for top-ranked enriched transcription factors (TFs). D qRT-PCR analysis of top scoring TFs in TLBR-2 and MAC2A cell lines. Each data represent mean ± SEM (n = 3). E Nuclear extract from TLBR-2 and MAC2A cells were tested for the presence of a multi-protein complex. Co-immunoprecipitation experiments were performed to evaluate binding of HELLS to YY1. Western blots are representative of two independent experiments. F ChIP qRT-PCR detection of YY1 in TLBR-2 HELLSKD and MAC2A HELLSKD after 48 h of doxycycline (DOX) induction. KLF4 and α-satellite were used as positive and negative controls, respectively. The values represent the fold enrichment over IgG (representative of three independent experiments).
