Fig. 1: The expression and effect of HNRNPD and ZHX2 on VM formation ability of glioma cells.

A Expression levels of HNRNPD in glioma tissues by western blot. Data are presented as the mean ± SD (n = 9, each group). **P < 0.01 vs. NBTs group; #P < 0.05 vs. LGGTs group. B Expression levels of HNRNPD in glioma cells by western blot. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. HA group. C–E CCK-8 assay, transwell, and three-dimensional culture were applied to evaluate the proliferation, migration, invasion, and tube formation effect of HNRNPD on U87 and U251 cells. Representative images and accompanying statistical plots were presented. The scale bar represents 50 µm. F Protein levels of MMP2, MMP9, and VE-cadherin regulated by HNRNPD in U87 and U251 cells. Representative protein expressions and corresponding IDVs of MMP2, MMP9, and VE-cadherin in U87 and U251 are shown. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. HNRNPD(−)-NC group. G Expression levels of ZHX2 in glioma tissues. Data are presented as the mean ± SD (n = 9, each group). *P < 0.05, **P < 0.01 vs. NBTs group; ##P < 0.01 vs. LGGTs group. H Expression levels of ZHX2 in glioma cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. HA group. I–K CCK-8 assay, transwell, and three-dimensional culture were applied to evaluate the proliferation, migration, invasion, and tube formation effect of ZHX2 on U87 and U251 cells. Representative images and accompanying statistical plots were presented. The scale bar represents 50 µm. L Protein levels of MMP2, MMP9, and VE-cadherin regulated by ZHX2 in U87 and U251 cells. Representative protein expressions and corresponding IDVs of MMP2, MMP9, and VE-cadherin in U87 and U251 are shown. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. ZHX2(+)-NC group.