Fig. 4: LINC01426 promotes LUAD progression through activating the hedgehog pathway. | Cell Death & Disease

Fig. 4: LINC01426 promotes LUAD progression through activating the hedgehog pathway.

From: Upregulation of LINC01426 promotes the progression and stemness in lung adenocarcinoma by enhancing the level of SHH protein to activate the hedgehog pathway

Fig. 4

A The number of colonies was analyzed in PC-9 and Calu3 cells treated with sh/Ctrl, sh/LINC01426#1, sh/LINC01426#1+NSC 228155, sh/LINC01426#1+IGF-1, sh/LINC01426#1+SAG, sh/LINC01426#1+LiCl, sh/LINC01426#1+Honokiol, respectively. One-way ANOVA. PC-9 and Calu3 cells were treated with sh/Ctrl, sh/LINC01426#1, or sh/LINC01426#1+SAG and used for subsequent rescue experiments. B EdU-positive cells were assessed by EdU assay. One-way ANOVA. C, D The apoptosis rate was evaluated by flow cytometry analysis and TUNEL assay. One-way ANOVA. E The migratory capacity was tested by transwell assay. One-way ANOVA. F The relative protein levels of E-cadherin and N-cadherin were detected by western blot. One-way ANOVA. G, H The RNA and protein levels of NANOG and OCT4 were examined by RT-qPCR and western blot in indicated cells. One-way ANOVA. I Sphere formation assay was conducted to test the efficiency of the sphere in indicated cells. One-way ANOVA. **P < 0.01.

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