Fig. 5: LINC01426 knockdown enhances the ubiquitination level of SHH protein. | Cell Death & Disease

Fig. 5: LINC01426 knockdown enhances the ubiquitination level of SHH protein.

From: Upregulation of LINC01426 promotes the progression and stemness in lung adenocarcinoma by enhancing the level of SHH protein to activate the hedgehog pathway

Fig. 5

A The relative levels of core proteins in the hedgehog pathway were evaluated in cells with LINC01426 knockdown. B The relative mRNA levels of SHH, Gli1, and Gli2 was evaluated by RT-qPCR. One-way ANOVA. One-way ANOVA. C The luciferase activity of SHH promoter was determined in cells transfected with shRNAs targeting LINC01426. One-way ANOVA. D, E The level of SHH protein was measured in PC-9 and Calu3 cells transfected with sh/LINC01426#1 after treatment with CHX for different time points. F SHH protein level in PC-9 and Calu3 cells treated with MG132 after transfected with sh/Ctrl or sh/LINC01426#1. G The ubiquitination level of SHH protein in PC-9 and Calu3 cells transfected with sh/LINC01426#1 or sh/Ctrl. n.s. no significance.

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