Fig. 6: Poly(I:C) treatment during differentiation results in downregulation of myelin genes and defects in reaching the more mature phenotype.

A–B qPCR analysis of Mbp and Plp1 in primary OLs treated with Poly(I:C) (PIC) 50 ug/ml for 72 h in differentiation (Diff) medium (results are the mean of four independent experiments performed in triplicate ±SEM, **P < 0.01, ***P < 0.001 One-way Anova followed by Bonferroni’s multiple comparison test). C–D’ Immunofluorescent staining for MBP. To evaluate MBP morphology coloured images (C, D) have been transformed in binary with ImageJ (C’, D’). E–G Histograms reported the percentage of MBP+ cells (E), measure of the cell area (F, delimited with yellow line in C’ and D) or of the area covered by MBP immunostaining (G, white signal in C’ and D’; data are mean ± SEM, n = 9 coverslips for each condition from three independent experiments, **P < 0.01, ***P < 0.001 Unpaired t-test). Scale bar 100 μm.