Fig. 5: Glucose-induced vascular constriction was impaired in Mypt1^SMKO mice.

A Expressions of proteins in MLCP signaling in VSMCs isolated from WT and Mypt1^SMKO mice. B ΔΨ_m in response to glucose (30 mM), Glu-Ins, NH₄^+, and Ch₃COO^- in VSMCs in isolated aortas from WT and Mypt1^SMKO mice. Scale bar, 50 μm. ΔΨ_m was detected by TMRM. C Vascular tone in response to glucose (30 mM) and Glu-Ins challenges in isolated aortas from WT and Mypt1^SMKO mice. n = 6. D Vascular tone in response to NH₄⁺ and Ch₃COO^- challenges in isolated aortas from WT and Mypt1^SMKO mice. n = 6. E ΔΨ_m in response to glucose (30 mM) in cultured VSMCs isolated from WT and Mypt1^SMKO mice. n = 5. F MLC phosphorylation in response to glucose (30 mM) in cultured VSMCs isolated from WT and Mypt1^SMKO mice. n = 5. Error bars represent SEM. ^*P < 0.05. ^**P < 0.01.