Fig. 4: PARP1 expression inhibits senescence induced by PLA2R1.
From: PLA2R1 promotes DNA damage and inhibits spontaneous tumor formation during aging
E–F MRC5 cells were infected with control, PLA2R1-, or PARP1-encoding retroviral vectors as indicated, and selected. A Fourteen days after seeding, cells were fixed and stained by crystal violet to visualize cell density. B The growth curve was performed by counting the number of cells and calculating the population doublings at each cell passage. Representative growth curves from three experiments with SD are shown. C RNA was prepared and RTqPCR performed against the proliferation marker Ki67 and normalized against GAPDH levels (n = 3). D Cells were fixed and a SA‐β‐galactosidase assay was performed. Percentage of SA-β-Gal positive cells was calculated and representative images are shown (n = 3). E, F RTqPCR performed against p21 (E) and IL8 (F) and normalized against GAPDH levels (n = 3). Graphs C–F show mean between three experiments ± SEM and p value was calculated using Student’s t-test. *p < 0.05.
