Table 2 Conditions for Immuno Histochemistry.
Platform | Primary antibody | Source | Primary concentration | Retrieval | Primary incubation | Secondary antibody | Secondary incubation | Visualisation system |
|---|---|---|---|---|---|---|---|---|
Ventana | Cleaved caspase 3 | Cell Signalling, ASP175 | 1:300 | CC1, pH 9, 32 min, 97 °C | 52 min | Omnimap anti-rabbit HRP | 12 min | ChromoMap DAB |
Dako | p53 | Dako, clone DO-7 | 1:3000 | Flex TRS High, pH 9, 20 min | 20 min | Flex HRP | 20 min | Flex DAB |
Leica Bond RX, refine kit | RCP (Rab11FIP1) | Cell Signalling | 0.15 µg/mL (1:100) | ER1, pH 6, 20 min, 98 °C | 30 min | Flex HRP rabbit | 20 min | Flex DAB |
