Fig. 2: Sham surgery and unilateral nephrectomy are associated with a lysosomal defect.
From: Surgical procedures suppress autophagic flux in the kidney
Mice underwent either no surgical manipulations (NORM), sham surgery (SHAM), or unilateral nephrectomy (UNX) and after 2 h the contralateral kidney was harvested. A qPCR analysis of TFEB-downstream genes Tfeb, Atp6v0d2, and Lamp2. B Representative proximal tubular cell images of TFEB nuclear versus cytoplasmic localization with quantification showing readings in duplicate for each mouse (Scale bar = 5 µm). C Immunoblot analysis for STX17, pGSK3βSer9, GSK3β, pAktSer308 and Akt. D Immunoblot analysis for TFEB in nuclear extracts from rapamycin -treated (RAPA) mice. E Immunoblot analysis for TFEB in nuclear extracts from NORM, SHAM and UNX mice. F Immunoblot analysis for pTFEBS142 in whole kidney extracts from NORM, SHAM and UNX mice. Quantification is corrected for GAPDH unless otherwise indicated. +P = 0.07, *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.0001. RDU = relative densitometry units corrected for GAPDH. H3 = Histone H3.
