Fig. 5: Both sham surgery and bilateral renal ischemia/reperfusion (24 h) suppressed autophagic flux in the kidney and heart. Autophagic flux normalizes at 72 h.
From: Surgical procedures suppress autophagic flux in the kidney
Mice underwent either no surgical manipulations, sham surgery (SHAM), or bilateral renal ischemia/reperfusion (I/R). After 24 h or 72 h, each mouse was treated with either vehicle or BafA1 (BAF) and after 26 h or 74 h of reperfusion, the contralateral kidneys and heart were harvested. Immunoblot analysis of LC3-II and p62 in the kidney (A) and the heart (B) with representative densitometry is demonstrated. RDU = relative densitometry units corrected for GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001.
